Environmental DNA in water
The vast majority of people who
have heard of environmental DNA (e-DNA) usually associate this with water
samples. By filtering water through a fine-mesh filter, we can collect DNA
residues floating around in the water, and with genetic analyses we can
determine which species it comes from.
NINA offers our own e-DNA kit for
taking water samples in rivers and lakes. With this simple kit, anyone can
collect e-DNA samples they submit for analysis at NINA. If you want to use our
e-DNA analyses, please contact Frode Fossøy or Rolf Sivertsgård.
We use species-specific markers to
detect individual species and general-species markers to describe the species
diversity of species groups in the aquatic environment. Species-specific
analyses use a genetic marker that is only found in the species we are looking
for using a qPCR or digital-PCR machine. General-species analyses use a genetic
marker found in many species, and we often want to investigate a specific group
such as fish, amphibians, insects or mammals. We then use a DNA metabarcoding analysis
(DNA metabarcoding) where we read millions of short DNA fragments that we then
compare with a reference database to determine which species each DNA fragment
originates from.